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Availability details
LS66 · FastGene® Scriptase III, cDNA synthesis kit with 10X RT buffer, MgCl₂, DTT, dNTPs, RNase-free water, oligo(dT) primers, random hexamers and RNase inhibitor, 100 reactions
Available quantity : 0
The stock indication is not contractual: this data is constantly changing and may vary depending on the quantities requested.
Bioreactive
LS66
LS66 · FastGene® Scriptase III, cDNA synthesis kit with 10X RT buffer, MgCl₂, DTT, dNTPs, RNase-free water, oligo(dT) primers, random hexamers and RNase inhibitor, 100 reactions
Three reverse transcriptases suited to different cDNA synthesis requirements
• M-MLV–derived reverse transcriptases for cDNA synthesis from RNA
• Available as reverse transcriptase with reagents, cDNA synthesis kit, or 5X ReadyMix, depending on the range
Additional information:
Sample profile: RNA with complex secondary structures and low abundance, enabled by enhanced thermostability
Activity temperature: 37 – ≈55 °C
Maximum cDNA length: ≈12 kb
Applications: RT-qPCR; first-strand cDNA synthesis for NGS and cDNA library construction
Available in three formats:
- Scriptase III cDNA synthesis reagents: reverse transcriptase with 5X First-Strand buffer, DTT, dNTPs and RNase-free water; primers and RNase inhibitor supplied separately
- cDNA synthesis kit: reverse transcriptase, 10X RT buffer, MgCl₂, DTT, dNTPs, RNase-free water, oligo(dT) primers, random hexamers and RNase inhibitor; only RNA sample needs to be added
- 5X ReadyMix: reverse transcriptase, RT buffer, dNTPs and RNase inhibitor premixed; oligo(dT) primers and random hexamers provided separately to be added with the RNA and required water
• M-MLV–derived reverse transcriptases for cDNA synthesis from RNA
• Available as reverse transcriptase with reagents, cDNA synthesis kit, or 5X ReadyMix, depending on the range
Additional information:
Sample profile: RNA with complex secondary structures and low abundance, enabled by enhanced thermostability
Activity temperature: 37 – ≈55 °C
Maximum cDNA length: ≈12 kb
Applications: RT-qPCR; first-strand cDNA synthesis for NGS and cDNA library construction
Available in three formats:
- Scriptase III cDNA synthesis reagents: reverse transcriptase with 5X First-Strand buffer, DTT, dNTPs and RNase-free water; primers and RNase inhibitor supplied separately
- cDNA synthesis kit: reverse transcriptase, 10X RT buffer, MgCl₂, DTT, dNTPs, RNase-free water, oligo(dT) primers, random hexamers and RNase inhibitor; only RNA sample needs to be added
- 5X ReadyMix: reverse transcriptase, RT buffer, dNTPs and RNase inhibitor premixed; oligo(dT) primers and random hexamers provided separately to be added with the RNA and required water
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Variant(s)
Sample profile: High amounts of RNA; low-complexity matrices
Activity temperature: 37–42 °C
Maximum cDNA length: ≈5 kb
Applications: end-point RT-PCR
Available in two formats:
- Scriptase Basic cDNA synthesis reagents: reverse transcriptase with 10X buffer, dNTPs and RNase-free water; primers and RNase inhibitor to be supplied separately
- Basic cDNA synthesis kit: additionally contains oligo(dT) primers, random hexamers and an RNase inhibitor; only the RNA sample needs to be added
Sample profile: Low amounts of RNA, synthesis of long cDNA
Activity temperature: 37–42 °C (ReadyMix Scriptase II: reverse transcription at 42 °C only)
Maximum cDNA length: ≈12 kb
Applications: RT-PCR; cDNA synthesis for NGS library preparation
Available in four formats:
- Scriptase II cDNA synthesis reagents: reverse transcriptase with 5X buffer, DTT, dNTPs and RNase-free water; primers and RNase inhibitor to be supplied separately
- cDNA synthesis kit: additionally contains oligo(dT) primers, random hexamers and an RNase inhibitor; only the RNA sample needs to be added
- ReadyMix 5X: contains random hexamers, an RNase inhibitor and an auxiliary protein; add RNA and RNase-free water
- ReadyMix 5X with oligo(dT): same composition with additional oligo(dT) primers; add RNA and RNase-free water
Sample profile: RNA with complex secondary structures and low abundance, enabled by enhanced thermostability
Activity temperature: 37 – ≈55 °C
Maximum cDNA length: ≈12 kb
Applications: RT-qPCR; first-strand cDNA synthesis for NGS and cDNA library construction
Available in three formats:
- Scriptase III cDNA synthesis reagents: reverse transcriptase with 5X First-Strand buffer, DTT, dNTPs and RNase-free water; primers and RNase inhibitor supplied separately
- cDNA synthesis kit: reverse transcriptase, 10X RT buffer, MgCl₂, DTT, dNTPs, RNase-free water, oligo(dT) primers, random hexamers and RNase inhibitor; only RNA sample needs to be added
- 5X ReadyMix: reverse transcriptase, RT buffer, dNTPs and RNase inhibitor premixed; oligo(dT) primers and random hexamers provided separately to be added with the RNA and required water
The stock indication is not contractual: this data is constantly changing and may vary depending on the quantities requested.