LS63 · FastGene® Scriptase II cDNA synthesis kit with 5X buffer, DTT, dNTPs, RNase-free water, oligo(dT) primers, random hexamers and RNase inhibitor, 100 reactions

Name: FastGene® Scriptase II cDNA synthesis kit with 5X buffer, DTT, dNTPs, RNase-free water, oligo(dT) primers, random hexamers and RNase inhibitor, 100 reactions
Brand: Nippon genetics
SKU: LS63
Price: 390.00 EUR

Sales Unit: 1

Brand: Nippon genetics

Part Number: LS63

Delivery time on request

Shipping costs with dry ice - flat rate per shipment

50.00 €

390.00 €

Three reverse transcriptases suited to different cDNA synthesis requirements

• M-MLV–derived reverse transcriptases for cDNA synthesis from RNA
• Available as reverse transcriptase with reagents, cDNA synthesis kit, or 5X ReadyMix, depending on the range

Additional information:

Sample profile: Low amounts of RNA, synthesis of long cDNA
Activity temperature: 37–42 °C (ReadyMix Scriptase II: reverse transcription at 42 °C only)
Maximum cDNA length: ≈12 kb
Applications: RT-PCR; cDNA synthesis for NGS library preparation

Available in four formats:
- Scriptase II cDNA synthesis reagents: reverse transcriptase with 5X buffer, DTT, dNTPs and RNase-free water; primers and RNase inhibitor to be supplied separately
- cDNA synthesis kit: additionally contains oligo(dT) primers, random hexamers and an RNase inhibitor; only the RNA sample needs to be added
- ReadyMix 5X: contains random hexamers, an RNase inhibitor and an auxiliary protein; add RNA and RNase-free water
- ReadyMix 5X with oligo(dT): same composition with additional oligo(dT) primers; add RNA and RNase-free water

Gross weight0,04 kg

Gross dimensions15,00 x 6,00 x 2,50 cm

UNSPSC Code41105500

523351 - technical Note

523351 - Safety data

523351 - Manufacturer data sheet

Variant(s)

Sample profile: High amounts of RNA; low-complexity matrices
Activity temperature: 37–42 °C
Maximum cDNA length: ≈5 kb
Applications: end-point RT-PCR

Available in two formats:
- Scriptase Basic cDNA synthesis reagents: reverse transcriptase with 10X buffer, dNTPs and RNase-free water; primers and RNase inhibitor to be supplied separately
- Basic cDNA synthesis kit: additionally contains oligo(dT) primers, random hexamers and an RNase inhibitor; only the RNA sample needs to be added

Available in stock: {0}

Supply in progress

Delivery time on request

Sample profile: Low amounts of RNA, synthesis of long cDNA
Activity temperature: 37–42 °C (ReadyMix Scriptase II: reverse transcription at 42 °C only)
Maximum cDNA length: ≈12 kb
Applications: RT-PCR; cDNA synthesis for NGS library preparation

Available in four formats:
- Scriptase II cDNA synthesis reagents: reverse transcriptase with 5X buffer, DTT, dNTPs and RNase-free water; primers and RNase inhibitor to be supplied separately
- cDNA synthesis kit: additionally contains oligo(dT) primers, random hexamers and an RNase inhibitor; only the RNA sample needs to be added
- ReadyMix 5X: contains random hexamers, an RNase inhibitor and an auxiliary protein; add RNA and RNase-free water
- ReadyMix 5X with oligo(dT): same composition with additional oligo(dT) primers; add RNA and RNase-free water

Available in stock: {0}

Supply in progress

Delivery time on request

Sample profile: RNA with complex secondary structures and low abundance, enabled by enhanced thermostability
Activity temperature: 37 – ≈55 °C
Maximum cDNA length: ≈12 kb
Applications: RT-qPCR; first-strand cDNA synthesis for NGS and cDNA library construction

Available in three formats:
- Scriptase III cDNA synthesis reagents: reverse transcriptase with 5X First-Strand buffer, DTT, dNTPs and RNase-free water; primers and RNase inhibitor supplied separately
- cDNA synthesis kit: reverse transcriptase, 10X RT buffer, MgCl₂, DTT, dNTPs, RNase-free water, oligo(dT) primers, random hexamers and RNase inhibitor; only RNA sample needs to be added
- 5X ReadyMix: reverse transcriptase, RT buffer, dNTPs and RNase inhibitor premixed; oligo(dT) primers and random hexamers provided separately to be added with the RNA and required water

Available in stock: {0}

Supply in progress

Delivery time on request

You may be also interested in these categories

Protein extraction and purification qPCRReagent for Extraction and purification of RNA, DNA and PCR products Transfection reagent Nucleic acid quantification Other enzyme for molecular biology Reagent for PCR Sequencing reagent Enzyme and DNA modification kit Stability and conservation of RNA Enzyme for PCR Reagent for molecular biology Reagent for NGS Restriction endonucleases Agarose for nucleic acid electrophoresis Nucleotide Protein Blotting reagent - antibody Buffer and other reagents for protein electrophoresis Expression, cloning, and functional analysis Reagent for RT-PCR Precast gel for protein electrophoresis DNA size marker Reagent for IEF dNTP - deoxyNucleoside TriPhosphate Staining reagent for electrophoresis Buffer for nucleic acid electrophoresis Amplification reagent for molecular biology Enzyme for qPCR Nucleic acid labeling Precast gel for nucleic acid electrophoresis Water for molecular biology Acrylamide for protein electrophoresis Buffer - amplification Protein detection and analysis dosage Nucleic acid preparation Protein size marker Blotting reagent Protein detection and analysis Protein dye for electrophoresis PCR kit and reagent Columns and protein purification reagents Cas9 reagent Surface decontamination - molecular biology Reagent for RT-qPCR PCR grade water Nucleic acid electrophoresis and revelation Stability and conservation of DNA Reagent for LAMP-PCR Solvent and chemical for molecular biology Lysis and protein extraction reagent Enzyme for RT-PCR Reagent for RT-LAMP Gene Primer Protein electrophoresis Gene editing kit Dye for molecular biology Blotting reagent

The stock indication is not contractual: this data is constantly changing and may vary depending on the quantities requested.