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Ampliqon : What reagents prêts tothe emploi for PCR and the qPCR?

Ampliqon designs and manufactures in Denmark PCR and qPCR reagents built for labs that want to get straight to the result. From Taq DNA Polymerase to TEMPase Hot Start, via ready-to-use Master Mix — including the RED version for direct loading — the brand blends simplicity, performance and rock-solid lot-to-lot consistency. Formats with or without ROX, clear documentation and hands-on support: Ampliqon streamlines workflows in research, QC and teaching, without compromising robustness.

A Danish PCR specialist

Founded by specialists in enzymology and formulation, Ampliqon set itself a clear mission: to make PCR more reliable, simpler and more reproducible. From its earliest developments, the brand invested in optimised polymerases and ready-to-use formulations that secure results at the bench. The launch of TEMPase Hot Start — a cold-inactive Taq designed to boost specificity — marked a turning point and established Ampliqon as a trusted partner to European laboratories. By prioritising manufacturing in Denmark, stringent quality control and responsive logistics, the company has steadily expanded its international footprint while maintaining hands-on technical support and a product culture focused on efficiency.

Expertise and areas of specialisation

Their core business: PCR/qPCR reagents that save time without compromising the amplification curve. For endpoint PCR, Ampliqon offers 2x Master Mix and high-performance Taq polymerases, with hot-start options to boost specificity or RED for direct gel loading - handy for routine screening and genotyping. Buffers are tuned to handle diverse sample matrices and challenging templates, with an excellent speed/fidelity balance.
For qPCR, the brand supplies SYBR Green mixes and probe-compatible formulations, offered with the ROX levels required by the main instruments. Performance is where it should be: clean starts, specific amplicons, linearity and efficiency that meet the standards of research and QC labs.
Ampliqon does not forget day-to-day use: clear protocols, comprehensive datasheets, pack sizes from exploratory tests to series runs, and lot-to-lot consistency backed by documented quality controls. Result: less optimisation, more usable data, whether in microbiology, molecular biology, food and beverage, environmental or pharmaceutical workflows. At Dutscher, what stands out most is their ready to succeed approach: robust, compatible reagents engineered to streamline the experimental workflow.

Ampliqon products supplied by Dutscher

Frequently asked questions about Ampliqon

Where are Ampliqon reagents manufactured?
In Denmark, with batch-to-batch control and Certificates of Analysis. Fast, well-controlled European production.

Standard Taq vs TEMPase Hot Start?
TEMPase remains inactive at low temperature and is activated at high temperature. Fewer primer-dimers and non-specific priming, greater specificity and sensitivity.

What is Master Mix RED used for?
Ready-to-use mix with loading dye: after PCR, load directly onto agarose gel; no additional loading buffer required.

qPCR compatibility and ROX levels?
Formulations without ROX, or with low or high ROX, to match major cyclers. See the data sheet for your instrument.

Storage/shipping conditions?
Store at ?20 °C; avoid freeze–thaw cycles. Short-term use at 4 °C may be possible depending on the product. Shipped under cold chain conditions.

Is technical support available?
Yes: guides, FAQs, protocols, and answers from PCR specialists to help you choose and optimise your reactions.