T752-1.0mg · Anti-Mouse TIM-3 (Clone B8.2C12) - Purified in vivo PLATINUM™ Functional Grade - 1.0 mg

TIM-3 is a member of the T cell immunoglobulin mucin gene family and encodes a type I
membrane protein consisting of an immunoglobulin variable-region-like domain, a mucin-like
domain, and a tyrosine phosphorylation motif1. TIM-3 functions as an important immune checkpoint receptor that helps regulate dendritic cell function2, T helper type I expansion, and induction of peripheral tolerance3. TIM-3 interacts with GAL-9, PtdSer, HMGB1 and CEACAM1 to activate biochemical pathways such as immune tolerance, T cell depletion, NF-κB signaling, and IL-2 secretion4. Additionally, TIM-3 expression correlates with terminal differentiation and exhaustion in tumors as well as chronic infection3. Due to its dysregulation in different types of cancer, TIM-3 blockade is being investigated as an anti-tumor immunotherapy2,4. TIM-3 also has potential as a prognostic marker in solid tumors4.

B8.2C12 was generated by immunizing Lewis and Lou/M female rats (Harlan Sprague-Dawley)
with Th1 polarized T cell clones and/or lines, including Th1-specific clone AE7 and in vitro
differentiated Th1 cell lines derived from 5B6 and DO11.10 T cell receptor transgenic mice1. Spleen cells were fused with myeloma cells and a large panel of monoclonal antibodies was screened on Th1 and Th2 cells by flow cytometry. B8.2C12 selectively stained Th1 cells. Gene expression cloning was then used to identify a complementary DNA, which was TIM-3.


- Product categories: All|Primary Monoclonal Antibodies
- Pkg Size: 1.0 mg
- Clone: B8.2C12
- Target: Tim-3
- Research Area: Cell Biology, Immunology, Immune Checkpoint
- Antigen Distribution: TIM-3 (CD366) is expressed on interferon-γ producing T cells, dendritic
cells, cytotoxic lymphocytes, exhausted T cells, natural killer cells, Th17, and myeloid cells.
TIM-3 is also expressed on CD8 + T cells in the tumor microenvironment as well as intratumoral
macrophages and monocytes. TIM-3 is expressed by T helper 1 cells after several rounds of
polarization in vitro. TIM-3 is not expressed by naïve T cells.

- Applications: FA, FC
- Concentration: ≥ 5.0 mg/ml
- Formulation: This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
- Storage and Stability: Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles.