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Lionheart FX automated microscope is a compact system for a broad range of imaging workflows. It offers up to 60x and 100x oil immersion magnification, with fluorescence, brightfield, color brightfield, and phase contrast channels for maximum application reach. An optional environment control cover provides incubation to 40 °C and effective containment for CO2/O2 control. A humidity chamber optimizes conditions for long-term live cell imaging applications, and an available dual reagent injector facilitates rapid kinetic assays.