GEN5SPOT · GEN5 SPOT COUNTING MODULE

The ability to accurately identify and count small objects, ranging in size from submicron to tens of microns in size, plays an important role in a number of life science research assays, such as steatosis, autophagy, liposomes, micronuclei, γH2AX foci, protein binding, fluorescence in situ hybridization, viral infection, and other assays with punctate biology. Knowing how many counted objects, or spots, are associated with a specific cell, organism, or other object aids in the identification of which may be responding to a specific treatment. Automating the identification and counting process can speed analysis, as well as improve accuracy by removing subjectivity from the process. The spot counting module available in Agilent BioTek Gen5 software enables the number of spots counted, and other information, to be tied to the original primary mask data, allowing these values to be known for each object initially identified in the image. This allows for responding cells or other objects to be identified within the image, based on established criteria, and normalized to the total number of objects identified. The module provides fast, robust data and can be used with Agilent BioTek Lionheart automated microscopes and Agilent BioTek Cytation cell imaging multimode readers.