G214-25ug · Anti-Human/Mouse/Rat Glycophorin A - PE

Glycophorins are transmembrane proteins of red blood cells (RBC)1 . There are four glycophorin encoding genes (A, B, C, and D), and a fifth pseudogene (E), which has no protein product.
Glycophorin A, B, and E are tandemly arranged repeats on chromosome 4 and are prone to
rearrangements that lead to rare blood groups. Glycophorin A and B carry MN and Ss blood group antigens.
Glycophorin A is a type I glycoprotein carrying sialylated O- and N-glycans, and most of the O-glycans are NeuAcα2-3Galβ1-3(NeuAcα2-6)GaINAc-tetrasaccharide chains linked to serine or
threonine residues 2 . The abundance of glycophorin A on the surface of RBCs is thought to create
a negatively charged cell surface of complex glycans that allows for RBC circulation without
sticking to other cells or blood vessel walls1 .
Glycophorin A binds CD170 3 (Siglec 5, which mediates sialic-acid dependent binding to cells4),
influenza virus, and EBA-175, the erythrocyte binding antigen of Plasmodium falciparum, the
parasite responsible for malaria in Africa 2 . Babesia divergens, which like Plasmodium is a
parasite in the Apicomplexa phylum, also uses glycophorins A and B to enter RBCs. Other
pathogens that bind to glycophorin A on the surface of RBCs include: some strains of
Escherichia coli, reoviruses (a group of viruses that includes rotavirus), encephalomyocarditis virus, and hepatitis A 2 . Glycophorin A also interacts with influenza virus but because influenza virus cannot replicate in anucleated RBCs glycophorin A might be acting as a decoy receptor to divert infection away from other tissues.
JC159 was generated using splenic hairy cell leukemia cells as immunogen and reacts with a
formalin-fixed, paraffin-embedded resistant epitope, likely between amino acids 27 and 40, on
the extracellular domain5.


- Product categories: All|Monoclonal Antibodies|Primary Monoclonal Antibodies>SurfaceTag Human CD Markers|Primary Monoclonal Antibodies>Surfacetag Mouse CD Markers
- Pkg Size: 25 µg
- Clone: JC159
- Target: Glycophorin A
- Research Area: Cell Adhesion, Immunology, Infectious Disease, Parasite, Viral
- Antigen Distribution: Glycophorin A (CD235a) is expressed on hematopoietic stem cells,
erythroid cells, and erythrocytes. Erythroleukemia can express glycophorin A on neoplastic
erythroblasts.
- Applications: FC, IF, IHC, IHC FFPE, WB
- Concentration: 0.2 mg/ml
- Formulation: This R-phycoerythrin (R-PE) conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
- Storage and Stability: This R-phycoerythrin (R-PE) conjugate is stable when stored at 2-8°C. Do not freeze.