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755197 · Hot FIREpol® EvaGreen® HRM 5X qPCR mix, without ROX, 5 x 1 ml, 1250 reactions

• High specificity and sensitivity
• Ready to use: just add matrix, water and primers
• Stable for 30 days at room temperature, long-term storage at -20 °C
Additional information:
• Highly sensitive mixture for accurate SNP detection
• Sensitive EvaGreen® dye for reliable HRM performance
• Hot start enzyme inactivated at room temperature, activation in 12 minutes at 95°C
• Contains: Hot FIREPol® DNA polymerase, 5X qPCR buffer with 12.5 mM MgCl2 (final concentration 2.5 mM), dNTPs, EvaGreen® dye and BSA
• Available with or without ROX
Variant(s)
• Ideal for all standard qPCR applications
• Hot start enzyme inactivated at room temperature, activation in 12 min at 95 °C
• Contains: Hot FIREPol® DNA polymerase, 5X qPCR buffer with 12.5 mM MgCl2 (final concentration 2.5 mM), dNTPs, EvaGreen® dye and BSA
• Available with or without ROX, or for capillary thermocyclers
• Ideal when the starting target is in low concentration
• Hot start enzyme inactivated at room temperature, activation in 10 min at 95 °C
• Contains: Hot FIREPol® DNA polymerase, 5X qPCR buffer with 12.5 mM MgCl2 (final concentration 2.5 mM), dNTPs and SolisGreen® dye
• Compatible with most ROX dependent and independent thermal cyclers
• Ideal when the sequence to be amplified is GC-rich
• High sensitivity even when the starting target is at low concentration
• Reduced risk of primer dimer formation
• Contains a blue dye to make pipetting easier
• Hot start enzyme inactivated at room temperature, activation in 12 minutes at 95°C
• Supplied with dUTPs to prevent cross-contamination after treatment with UNG (not supplied)
• Contains: Hot FIREPol® DNA polymerase, 5X qPCR buffer with 12.5 mM MgCl2 (final concentration 2.5 mM), dNTPs (including dUTP), EvaGreen® dye, GC enhancer and blue dye
• Compatible with most ROX dependent and independent thermal cyclers
• Highly sensitive mixture for accurate SNP detection
• Sensitive EvaGreen® dye for reliable HRM performance
• Hot start enzyme inactivated at room temperature, activation in 12 minutes at 95°C
• Contains: Hot FIREPol® DNA polymerase, 5X qPCR buffer with 12.5 mM MgCl2 (final concentration 2.5 mM), dNTPs, EvaGreen® dye and BSA
• Available with or without ROX



