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Availability details
455644 · Taq hot start Tempase master mix 5X lyophilised - 500 reactions
Available quantity : 0
The stock indication is not contractual: this data is constantly changing and may vary depending on the quantities requested.
455644 · Taq hot start Tempase master mix 5X lyophilised - 500 reactions
• Inactivated at room temperature, activated at 95°C for 15 minutes
• dUTP incorporation possible
• High specificity and sensitivity for high yield
• For detection of even low-abundance targets, screening, multiplexing, direct PCR on colonies, and qPCR applications
Sample available, contact us!
Additional information:
• Ready to use once reconstituted in the supplied buffer, adding only the template, primers and water
• Stable for 12 months at room temperature: storage and transport at room temperature
• Amplification up to 4 kb
• The reconstituted Taq hot start Tempase master mix (5X) is composed of Hot Taq DNA polymerase, dNTPs, NH4+ buffer, and MgCl2
• Available in a ‘Ready to load’ version, containing two dyes (orange G and xylene cyanol FF) and a loading buffer, for migration monitoring
• dUTP incorporation possible
• High specificity and sensitivity for high yield
• For detection of even low-abundance targets, screening, multiplexing, direct PCR on colonies, and qPCR applications
Sample available, contact us!
Additional information:
• Ready to use once reconstituted in the supplied buffer, adding only the template, primers and water
• Stable for 12 months at room temperature: storage and transport at room temperature
• Amplification up to 4 kb
• The reconstituted Taq hot start Tempase master mix (5X) is composed of Hot Taq DNA polymerase, dNTPs, NH4+ buffer, and MgCl2
• Available in a ‘Ready to load’ version, containing two dyes (orange G and xylene cyanol FF) and a loading buffer, for migration monitoring
Suggested application(s)PCR and nucleic acid amplification
455644 - Safety data455644 - Brochure455644 - Manufacturer data sheetVariant(s)
Taq Hot Start Tempase 5U/µl, with or without buffer and MgCl2:
• Hot Taq polymerase 5 U/µl, with or without 10X buffer and MgCl2
• To be supplemented with dNTPs, template, primers, water, as well as 10X buffer and MgCl2 if not supplied
• Several buffers available
• Ready-to-use master mix, only add template, primers and water
• The 2X master mix contains Hot Taq DNA polymerase, dNTPs, MgCl2 for a final concentration of 1.5 mM, and buffer 1 (KCl/(NH4)2 SO4 Tris) or buffer 2 ((NH4)2 SO4)
Available in a ‘Ready to load’ version, containing a loading buffer and a red dye to track migration:
• Can be deposited directly after PCR, on agarose or SDS gel
• No interference with PCR, the products obtained can be used for downstream applications (sequencing, etc.) after purification on a column such as PureIT Exo PCR ClearUp
• Ready-to-use master mix, only add template, primers and water
• For amplification of GC-rich DNA sequences
• Minimises the risk of artefacts due to possible non-specific polymerisation before the first denaturation step
• The 2X master mix contains Hot Taq DNA polymerase, optimised buffer, dNTPs, 3.0 mM MgCl2, and stabilising agents
Available with master mix I or II. :
• Master mix I is suitable in most cases. However, if the results obtained are not satisfactory, it is recommended to use master mix 2
• Contact us for samples to select the master mix that will optimise your results
• Hot Taq 5U/µl with glycerol-free storage buffer, without 10X buffer or 25 mM MgCl2
• For applications involving a lyophilisation step, or requiring precise pipetting of small volumes (manual or via automated pipetting) or automated high-throughput screening
• Several buffers available
• Ready to use once reconstituted in the supplied buffer, adding only the template, primers and water
• Stable for 12 months at room temperature: storage and transport at room temperature
• Amplification up to 4 kb
• The reconstituted Taq hot start Tempase master mix (5X) is composed of Hot Taq DNA polymerase, dNTPs, NH4+ buffer, and MgCl2
• Available in a ‘Ready to load’ version, containing two dyes (orange G and xylene cyanol FF) and a loading buffer, for migration monitoring
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The stock indication is not contractual: this data is constantly changing and may vary depending on the quantities requested.