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455534 · Taq master mix 2X, ready to load - MgCl2 2mM - 100 reactions

• Amplification up to 5 kb, compatible with TA cloning and dUTP incorporation
• 5' -> 3' exonuclease activity and no 3'-> 5' exonuclease activity
Additional information:
Ready-to-use master mix, add template, primers and water only
• Taq 2X master mix contains Taq DNA polymerase, NH4+ buffer, 0.4 mM of each dNTP, MgCl2 for a final concentration of 1.5 or 2 mM, and stabilising agents
• OptiMix Clear 2X master mix is supplied with a buffer for amplifications up to 4kb with increased specificity even with difficult templates
Available in a ‘ready to load’ version, containing a loading buffer and a red dye to track migration:
• Allows direct deposition after PCR, on agarose gel or SDS page
• No interference with PCR; the products obtained can be used for downstream applications (sequencing, etc.) after purification on a column such as PureIT Exo PCR ClearUp
Variant(s)
Taq 5 U/µl, with or without buffer and MgCl2:
• Taq polymerase 5 U/µl, with or without 10X buffer and MgCl2
• To be supplemented with dNTPs, template, primers, water, as well as 10X buffer and MgCl2 if not supplied
• Several buffers available
Ready-to-use master mix, add template, primers and water only
• Taq 2X master mix contains Taq DNA polymerase, NH4+ buffer, 0.4 mM of each dNTP, MgCl2 for a final concentration of 1.5 or 2 mM, and stabilising agents
• OptiMix Clear 2X master mix is supplied with a buffer for amplifications up to 4kb with increased specificity even with difficult templates
Available in a ‘ready to load’ version, containing a loading buffer and a red dye to track migration:
• Allows direct deposition after PCR, on agarose gel or SDS page
• No interference with PCR; the products obtained can be used for downstream applications (sequencing, etc.) after purification on a column such as PureIT Exo PCR ClearUp
Taq without glycerol:
• Taq 5 U/µl or 50 U/µl with glycerol-free storage buffer, with or without 10X buffer and 25 mM MgCl2
• For applications involving a lyophilisation step, or requiring precise pipetting of small volumes (manual or via automated pipetting)
• 1U allows an average of 500 reactions to be performed
• Several buffers available



