Sales Unit: 1
Brand: Biotium
Part Number: 30002
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50.00 €
365.00 €
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30002 · Viability/cytotoxicity test for live and dead animal cells - 300 tests
Available quantity : 0
The stock indication is not contractual: this data is constantly changing and may vary depending on the quantities requested.
BioreactiveCell labelling and analysisAnalysis of apoptosis and cell viabilityCell viability marker
30002
30002 · Viability/cytotoxicity test for live and dead animal cells - 300 tests
Stains live animal cells with green fluorescence and dead cells with red fluorescence for detection by fluorescence microscopy or flow cytometry
• Kit contenant 2 colorants : la calcéine AM et l'homodimère III d'éthidium
• Calcein-AM is a cell-permeable, non-fluorescent ester that can passively enter cells. In viable cells, it is hydrolysed by esterases in the cytoplasm to release the green fluorescent dye calcein. Calcein itself is impermeable to membranes and is only retained in viable cells with an intact plasma membrane
• Ethidium homodimer III is a red fluorescent nucleic acid dye that is impermeable to membranes. It selectively colours dead cells with damaged plasma membranes (necrotic or late apoptotic cells)
• Kit contenant 2 colorants : la calcéine AM et l'homodimère III d'éthidium
• Calcein-AM is a cell-permeable, non-fluorescent ester that can passively enter cells. In viable cells, it is hydrolysed by esterases in the cytoplasm to release the green fluorescent dye calcein. Calcein itself is impermeable to membranes and is only retained in viable cells with an intact plasma membrane
• Ethidium homodimer III is a red fluorescent nucleic acid dye that is impermeable to membranes. It selectively colours dead cells with damaged plasma membranes (necrotic or late apoptotic cells)
Suggested application(s)Immunofluorescence and cell labeling
Gross weight0,02 kg
UNSPSC Code12000000
Item subject to a restricted return policy.Click here for more information
462337 - Safety data462337 - Manufacturer data sheetVariant(s)
Stains live animal cells with green fluorescence and dead cells with red fluorescence for detection by fluorescence microscopy or flow cytometry
• Kit contenant 2 colorants : la calcéine AM et l'homodimère III d'éthidium
• Calcein-AM is a cell-permeable, non-fluorescent ester that can passively enter cells. In viable cells, it is hydrolysed by esterases in the cytoplasm to release the green fluorescent dye calcein. Calcein itself is impermeable to membranes and is only retained in viable cells with an intact plasma membrane
• Ethidium homodimer III is a red fluorescent nucleic acid dye that is impermeable to membranes. It selectively colours dead cells with damaged plasma membranes (necrotic or late apoptotic cells)
Membrane impermeable nucleic acid dyes can be used to selectively stain late necrotic and apoptotic cells with leaky membranes
• Colorants ont une très faible fluorescence jusqu'à ce qu'ils se lient à l'ADN ou à l'ARN, peuvent donc être utilisés pour colorer les cellules sans étape de lavage pour une imagerie homogène et en temps réel dans le milieu de culture cellulaire
• Specific counterstaining of the nucleus in fixed cells
• Convient à l'imagerie des cellules vivantes sur plusieurs jours
• NucSpot® 568/580: red cell impermeable dye for Cy®3 or PE channels
• NucSpot® 594/615: red cell impermeable dye for Texas Red® or PE-Texas Red® channels
NucSpot® Live 488 and NucSpot® Live 650 nucleic acid stains specifically stain the nuclei of living or fixed cells
• Présentent une excellente spécificité pour l'ADN sans nécessiter d'étape de lavage, avec une faible toxicité pour l'imagerie des cellules vivantes
• NucSpot® Live 650 compatible with super-resolution SIM and STED imaging
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The stock indication is not contractual: this data is constantly changing and may vary depending on the quantities requested.