Western Blot

Prepare →

Separate and blot →

Antibody incubation →

Detect ✓

Blotting Paper for Western Blotting


Azure Biosystems AC2110-AC2111 Blotting Paper

Azure Biosystems - AC2110-AC2111 Blotting Paper

Pre-cut blotting paper for Western blot transfer

Avoid measuring and cutting blotting paper to the size of your mini-gels. These pre-cut papers completely cover your gels and membranes.
 

  • 8x10cm gels – blotting papers are 9x13cm
  • 7x9cm gels – blotting papers are 8x10cm
Azure Biosystems

Transfer Buffer


Advansta FLASHBlot Transfer Buffer

Advansta - FLASHBlot Transfer Buffer

Shorten your experimental time considerably

Designed for faster, more efficient protein transfer with increased sensitivity. The FLASHBlot transfer buffer will shorten your experimental time considerably.
 

  • Faster
  • More sensitive for low abundance proteins

  • Better detection of post-translationally modified proteins

  • Better transfer

Advansta

Blotting Paper


Advansta Blotting Paper Sheets

Advansta - Blotting Paper Sheets

Pre-cut paper for electrophoretic transfers

This blotting paper is ideal for use in transfer sandwiches and cassettes with electrophoretic transfer.
 

  • Works with most mini gel transfer assemblies
  • Available in 7x9 and 8x10 cm

Advansta

Protein Sample Loading Buffers


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BioVision - 3X SDS-PAGE Loading Buffer

Ready-to-use buffer

BioVision's 3X SDS-PAGE Loading Buffer is a ready-to-use buffer solution for preparation of protein samples in SDS-PAGE. The buffer is also called Laemmli sample buffer.
 

  • 10 mL (5 x 2 mL)
  • Stored at -20 C°
BioVision

Protein Sample Loading Buffers


Advansta - Protein Sample Loading Buffer

Advansta - Protein Sample Loading Buffer

Pre-mixed standard Laemmli loading buffers for protein electrophoresis

Choose from reducing or non-reducing buffers. Standardized buffers helps you obtain reproducible results.
 

  • Pre-mixed - just add an equal volume to your protein sample (supplied as 2X)

  • Contain Bromophenol blue to enable visualization of the dye front and monitoring of the progress of electrophoresis

Advansta

Protein Sample Loading Buffers


Santa Cruz Biotechnology - Electrophoresis Sample Buffer, 2X

Santa Cruz Biotechnology - Electrophoresis Sample Buffer, 2X

Ready-to-use electrophoresis sample buffer

Electrophoresis Sample Buffer, 2X is a ready-to-use reducing electrophoresis sample buffer solution with bromophenol blue for preparation of protein samples in SDS-PAGE. Bromophenol is included as a tracking dye to monitor the sample during the electrophoretic run.
 

  • Ready-to-use
  • With bromophenol blue
  • Contains DTT and TCEP-HCl, which disulfide bonds to ensure proteins are fully denatured before loading to the gel
Santa Cruz Biotechnology

Protein Ladder


Santa Cruz Biotechnology - Cruz Marker™ Molecular Weight Standards and Prestained Molecular Weight Standards

Santa Cruz Biotechnology - Cruz Marker™ Molecular Weight Standards and Prestained Molecular Weight Standards

Consistent visibility on the final western blot image

Ready-to-use in SDS-PAGE loading buffer. Can be loaded directly into an SDS-PAGE gel.
 

  • Sufficient for 50 gels

  • Ladder consists of 6 bands (132, 90, 55, 43, 34, and 23 kDa)

  • Must be used in combination with Cruz Marker™ MW Tag detection antibodies

Santa Cruz Biotechnology

Membranes


Membranes

Santa Cruz Biotechnology  - Membranes

Membranes for your Western blot experiments

Santa Cruz Biotechnology offer both Nitrocellulose and PVDF membranes that are ideal for use in transfer sandwiches and cassettes with electrophoretic transfer.
 

  • UltraCruz® Nitrocellulose Pure Transfer Membranes 

  • UltraCruz® PVDF Transfer Membrane

  • Immobilon®-FL PVDF membrane

Santa Cruz Biotechnology

Protein Ladder


Biovision - BriteRuler™ Pre-stained Protein Ladder

Biovision - BriteRuler™ Pre-stained Protein Ladder

Ready-to-use protein ladder

Ready-to use formulation in gel loading buffer, requires no heating, dilution, or addition of reducing agents.
 

  • Red and green reference bands included in the ladder

  • 100 applications

  • Volume: 250 uL

BioVision

Gel Loading Tips


Santa Cruz - ExactaCruz® Round Gel Loading Tips

Santa Cruz - ExactaCruz® Round Gel Loading Tips

Excellent tips for loading samples on gels

Suitable for a wide variety of vertical-loading gel applications with a 0.57 mm O.D.
 

  • 83 mm long and a 5 μl graduation mark for quick volume check

  • Pipette tips are certified

  • RNase/DNase-free, human DNA, and PCR inhibitor-free and non-pyrogenic

  • Available in racks or in bulk bags

Santa Cruz

High-quality liquid handling tools for molecular biology research


Sorenson Bioscience - Tubes, plates, tips, etc.

Sorenson Bioscience - Tubes, plates, tips, etc.

High-quality liquid handling tools for molecular biology research

Pipette tips, PCR products, microcentrifuge tubes, and MultiFlex gel loading tips from leading manufacturer Sorenson Bioscience. A range of consumables of high quality.
 

  • Consistent quality performance

  • Low-binding surface technology

  • Certificate of analysis for each product

Sorensen