Real-Time PCR

Purification →

Sample Control →

Reagents →

Detect ✓

Real-Time PCR (qPCR) miRNA QRT-PCR 

Agilent - miRNA QRT-PCR Detection Kit

Agilent - miRNA QRT-PCR Detection Kit

Detect mature miRNA from as little as 15 ng of total RNA

The miRNA QRT-PCR and miRNA QRT-PCR Master Mix Detection Kits detect mature miRNA from as little as 15 ng of total RNA input on various sample types. The detection kits also differentiate between miRNAs that differ by as few as a single nucleotide. 

  • Detects mature miRNA

  • Differentiates miRNA that differ by a single nucleotide

  • Sensitive detection down to 10 copies

  • Highly accurate results in 3 hours

  • miRNA Specific Forward Primers

  • miRNA Primer Design Guidelines


Real-Time PCR (qPCR) cDNA Synthesis Kits

Agilent cDNA Synthesis Kit

Agilent - cDNA Synthesis Kit

cDNA Synthesis

Using the AffinityScript cDNA Synthesis Kit you are ensured efficient conversion of RNA to cDNA. It is fully optimized for real-time quantitative PCR (qPCR) applications and kit utilizes qPCR-grade AffinityScript Multiple Temperature Reverse Transcriptase. It includes a buffer system optimized for real-time qPCR.

  • Optimized for real-time quantitative PCR (qPCR) applications
  • Produces cDNA in 15 minutes with linear detection from 3 pg to 3 µg total RNA
  • Creates up to 12 kb cDNA in 25 minutes or less
  • Available as a first-strand synthesis kit and a two-step QRT-PCR master mix format

Real-Time PCR (qPCR) Master Mixes

Agilent Brilliant III qPCR Master Mixes

Agilent -  Brilliant III qPCR Master Mixes

Brilliant results with qPCR Master Mixes

Brilliant III ultrafast SYBR® qPCR and qRT-PCR Master Mixes provide specificity by utilizing a novel faster-activating hot start method. The hot start method minimizes the formation of primer-dimers and other off-target reactions. These reagents are robust and reproducible across many assays on fast cycling real-time platforms.

2-step/Brilliant III qPCR Ultra-Fast Master Mixes

  • Probe or SYBR® green assays from cDNA
  • Extremely fast runs while maintaining sensitivity and great resistance to qPCR inhibitors
  • Optimized protocol for most qPCR instruments

1-step/Brilliant III QRT-PCR Ultra-Fast Master Mixes

  • Perform qPCR directly on your mRNA
  • Gives you reverse transcriptase and qPCR in one step
  • Fast, reliable, and easy



Real-time PCR Kits

Takara Bio Premix Ex Taq Master mix probe-based real-time PCR

Takara Bio - Premix Ex Taq™

Master mix for probe-based real-time PCR

The Premix Ex Taq master mix is ideal for high-speed PCR, allows accurate target quantification, and enables highly reproducible and realiable PCR analyses.

  • Rapid and accurate PCR product detection
  • Quantitative gene expression analysis using real-time PCR (qPCR)
  • 2X master mix enables easy pipetting
  • Optimized buffer increases amplification efficiency, highly sensitive probe-based qPCR assays, and 5' nuclease assays
  • Included Tli RNase H minimizes PCR inhibition by removing residual mRNA in input cDNA
Takara Bio

Real-time PCR Kits

Takara Bio TB Green Premix Ex Taq™ (Tli RNase H Plus)

Takara Bio - TB Green® Premix Ex Taq™ (Tli RNase H Plus)

qPCR with TB Green detection 

Designed for high-speed, high-sensitivity qPCR. The premix limits PCR inhibition from residual mRNA and provides superior specificity and fidelity compared to conventional Taq DNA Polymerase.

  • Lower Cq values as a result of fast extension rates
  • Use with broad range of amplicon sizes, even over 500 bp
  • Convenient 2X master mix is excellent for high-speed qPCR
  • High sensitivity: detects fewer than 100 copies
  • Hot-start PCR enzyme enables high efficiency and sensitivity in real-time PCR (qPCR)
  • Compatible with all commonly used real-time PCR (qPCR) instruments
  • Minimizes qPCR inhibition due to residual mRNA in input cDNA
  • Includes TB Green for intercalator-based qPCR
Takara Bio

Real-time PCR Kits

Takara Bio - One-Step TB Green® PrimeScript™

Takara Bio - One-Step TB Green® PrimeScript™ RT-PCR Kit II (Perfect Real Time)

qPCR with TB Green detection

For intercalator-based, one-step RT-qPCR. Includes TB Green detection, extension of longer templates and GC-rich RNA, optimized buffer for high specificity, and high-efficiency hot-start PCR enzyme.

  • Quantitative real-time PCR enables rapid and accurate analysis of RNA from viral, rare, or precious samples
  • The One-Step TB Green PrimeScript RT-PCR Kit II (Perfect Real Time) buffer system is optimized for quantitative real-time PCR, allowing efficient amplification and highly sensitive detection
  • One-Step TB Green RT-PCR Buffer IV is a 2X TB Green premix; the premix formulation simplifies reaction mixture preparation
Takara Bio

Real-time PCR Kits

Takara Bio - One Step PrimeScript III RT-PCR Kit

Takara Bio - One Step PrimeScript™ III RT-PCR Kit

qPCR with probe detection

A master mix for performing one-step real-time, probe-based RT-qPCR in a single tube using a fast, easy protocol.

  • Fast, easy one-step RT-qPCR in a single tube using probe-based detection
  • Efficient, specific cDNA synthesis and amplification, also from complex RNA templates
  • Suitable for a wide variety of samples from different sources, including crude RNA extracts
  • Quick and simple protocol reduces the risk of contamination
Takara Bio

Reverse Transcription Prior to qPCR

Takara Bio  PrimeScript RT Master Mix (Perfect Real Time)

Takara Bio - PrimeScript™ RT Master Mix (Perfect Real Time)

Convenient master mix for real-time PCR

Allows fast, efficient cDNA template synthesis for real-time PCR. The PrimeScript RT Master Mix enables full-length cDNA synthesis for real-time PCR in just 15 minutes.

  • First-strand cDNA synthesis for real-time RT-PCR (qPCR)
  • Two-step real-time RT-PCR
  • Reverse transcription in just 15 minutes
  • Master mix format reduces pipetting steps and the risk of contamination
  • Works efficiently with GC-rich and/or higher-structured RNA templates
  • Synthesizes full-length cDNA with high accuracy and at high yield prior to qPCR analysis
Takara Bio

Multiplex Gene Expression Assay

eBioscience - QuantiGene™ Plex

eBioscience - QuantiGene™ Plex

Multiplexed gene expression quantitation

Accurate and precise RNA quantitation of up to 80 genes in a single well.

  • Multiplex and customizable RNA or DNA measurement using a Luminex® instrument
  • No need for RNA purification or target amplification - just lyse and run
  • Works with difficult sample types, including FFPE, and directly with blood

Preamp and Reverse Transcription Master Mixes

Fluidigm - Master Mixes Preamp and Reverse Transcription Master Mixes

Fluidigm - Master Mixes

Preamp and Reverse Transcription Master Mixes

Fluidigm delivers optimized Preamp and Reverse Transcription Master Mixes for the Biomark and Biomark HD platforms. Preamplification allows for multiplex amplification of up to 96 targets. A pool of primers is prepared from the same gene expression assays to be used for qPCR. By using the real-time qPCR assays in the preamplification reaction, only the targets of interest are amplified. A limited number of cycles is used and the cDNA is amplified without significant bias.

  • Optimized for small volumes
  • Optimized for TaqMan Gene Expression Assays

Integrated Fluidic Circuits (IFCs)

Fluidigm - Biomark HD IFCs

Fluidigm - Biomark HD IFCs

Make up to 9.216 datapoints in one run

Enhance your productivity with high-performance solutions for robust processing of samples in nanoscale reactions, enabling quick and reliable gene expression, copy count, and digital PCR. The revolutionary integrated fluidic circuits (IFCs) enable automated molecular biology that significantly minimizes pipetting steps, hands-on time, and reagent consumption while maximizing efficiency, cost savings, and robust data production.

  • Find the format that covers your project from 12-192 samples combined with 12-192 assays
  • Sample.Assays:
  • 192.24
  • 96.96
  • 48.48
  • 24.192
  • 12.12*6